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Fig. 3 | BMC Ophthalmology

Fig. 3

From: MiR-221/SIRT1/Nrf2 signal axis regulates high glucose induced apoptosis in human retinal microvascular endothelial cells

Fig. 3

MiR-221 targeted and regulated SIRT1 expression. a: Bioinformatics Analysis (StarBase: http://starbase.sysu.edu.cn/) of the Binding Sites of miR-221 and SIRT1. b: Luciferase reporter assay in AGS cells transfected with wild type or mutated SIRT1 luciferase reporter system. (* indicates P < 0.05; ** indicates P < 0.01). c: qRT-PCR analysis of miR-221 level in hRMEC transfected with miR-221 inhibitor or mimics as indicated. (* indicates P < 0.05; ** indicates P < 0.01). d: WB detection of SIRT1 expression in hRMEC transfected with miR-221 inhibitor or mimics as indicated. Full-length blots are presented in Supplementary Figure 3D.Statistical analysis of relative expression was conveyed and displayed in columns. (* indicates P < 0.05)

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