Fig. 5

MiR-221 regulates HG induced apoptosis of hRMEC cells through SIRT1/Nrf2 pathway. a: hRMEC transfected with p-SIRT1 and/or miR-221 mimics, treatment with ML385 as indicated were cultured under HG condition. Viability of these cells was detected by MTT assay. (* indicates P < 0.05; ** indicates P < 0.01). b: hRMEC transfected with p-SIRT1 and/or miR-221 mimics, treatment with ML385 as indicated were cultured under HG condition. Apoptosis of these cells were detected by FACS assay. Statistical analysis was conveyed and displayed in columns. (* indicates P < 0.05; ** indicates P < 0.01). c: hRMEC transfected with p-SIRT1 and/or miR-221 mimics, treatment with ML385 as indicated were cultured under HG condition. Protein level of cleaved-caspase3, Bcl-2 and Bax were detected by Western Blot. Full-length blots are presented in Supplementary Figure 5C.Statistical analysis was conveyed and displayed in columns. (* indicates P < 0.05; ** indicates P < 0.01)