Fig. 2

Flow cytometry of vitreous sample. The plasma cells are shown in red and other cells in black. The analyses in Panels a-c were completed 3 h after sample collection, and the analyses in Panels d-f were completed 24 h later. 2000–4000 total events were acquired in each tube. Event numbers were limited by the cells present in the available sample. Plasma cells were approximately 5% of events. Samples were analyzed on a FACSCanto II (Becton Dickinson, San Jose, CA). Events were acquired in FACSDiva software (BD) and analyzed in Kaluza software (Beckman Coulter, Brea, CA). Panels A-C: Cells were stained with a cocktail of CD45-V500, CD19-V450, κ-FITC, λ-PE, CD20-PerCP, CD10-PE-Cy7, CD11c-APC and CD3-APC-H7 as described (Tran DN et al. 2016). a: All cells are shown. The plasma cells have moderate side scatter and are moderately bright for CD45. b and c: only the cells in the gate in Panel a are shown. b: The plasma cells are CD19+ and CD20-. c: The plasma cells are surface lambda positive. Panels d-f were stained with CD45-V500, CD38-V450, intracellular κ-FITC, intracellular λ-PE,CD20-PerCP, CD56-PE-Cy7, CD138-APC and CD19-APC-H7 as described (Tran et al. 2016). d: All cells are shown. The plasma cells are very bright for CD38. e and f: only the cells in the gate in Panel d are shown. e: The plasma cells are substantially CD138+. f: The plasma cells are CD56 negative