Fig. 1

a-Haematoxylin and Eosin (H&E) stained section of trabecular meshwork. The arrow points to compressed trabecular beams. The asterisk indicates the spaces created by dispersion of the trabecular beams from surgical sampling. b-Periodic acid-Schiff (PAS) stain of the trabecular meshwork. The beams are stained pink. The arrow points to a layer of darker pink material on the trabecular beams indicating trabecular meshwork cell basement membrane. c-PAS stain showing the presence of Descemet’s membrane exhibiting Hassall-Henle excrescences. d-H&E stained trabecular meshwork from eye number 6 with pigment dispersion glaucoma. Note the dark brown pigment deposited on the trabecular beams (arrow). e-Elastin Von Gieson stain of trabecular meshwork showing patchy elastin presence (arrows point to black linear elastin). f-Collagen IV immunohistochemistry. The arrow points to tram track linear staining (brown-positive) over the beams.