Table 2 Primer sequences of the selected DEGs
From: RNA sequencing and bioinformatics analysis of human lens epithelial cells in age-related cataract
Gene Symbol | Forward primer | Reverse primer | Product length | Tm |
|---|---|---|---|---|
β-ACTIN | CATTCCAAATATGAGATGCGTT | TACACGAAAGCAATGCTATCAC | 133 | 60 |
HSF4 | TGCAGGAGGCAAGAGAAAG | AGAAGGGCACCAGGTAGA | 99 | 60 |
LAMA4 | ACCTCGGGATACTGCACA | ATTTCTCCGCTGACATCCA | 126 | 60 |
NLRP3 | AAAGGAAGTGGACTGCGA | CCAGCAGGTAGTACATGG | 83 | 60 |
CRYBA2 | TCCGGAGAGTCCAGCACTA | GCAGGAAGATTCAGGAACC | 81 | 60 |
NRCAM | AGGGATGGCGAAGAATGA | CCTCTTTGCACAACTGCATA | 87 | 60 |
TGIF1 | CTGATGATTACCAAGAGGCG | TGATGTGCCCTACGAAGC | 85 | 60 |
ID11 | GGGCAAGAGGAATTACGTG | CCAGGCTGGATGCAGTTA | 110 | 60 |
ANGPTL4 | CTCAGTCACATTGACTGACG | GGACCTACACACAACAGC | 83 | 60 |
DUSP1 | CTGAACTCAGCACATTCGG | GGATCACACACTGAGTCCT | 93 | 60 |
CAV2 | GCCAGGATTGAATACTTGGAC | TGAACAGAACAGTGGTGCT | 93 | 60 |