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Fig. 4 | BMC Ophthalmology

Fig. 4

From: PI3K/AKT/mTOR signaling participates in insulin‐mediated regulation of pathological myopia‐related factors in retinal pigment epithelial cells

Fig. 4

The inhibitor of PI3K LY294002 acted antagonistic effect on insulin. a The protein levels of main molecules in the PI3K/AKT/mTOR signaling pathway were measured by western blotting, *p < 0.05, **p < 0.01, ***p < 0.001 vs.. the control group, ### p < 0.0501, vs.. the 24 h treatment group. Transcriptional levels of (b) TIMP-2, (c) MMP-2, (d) bFGF, and (e) IGF-1 were detected by RT-qPCR in ARPE-19 cells treated with 1 µg/ml of insulin or LY294002 for 72 h. Protein expression levels of (f) TIMP-2, (g) MMP-2, (h) bFGF, and (i) IGF-1 were detected by ELISA in ARPE-19 cells treated with 1 µg/mL of insulin or LY294002 for 72 h. *p < 0.05, **p < 0.01, ***p < 0.001 vs.. the blank group; ##p < 0.01, ###p < 0.001 vs.. the Insulin group

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