Table 2 Primers Used for COL5A1 Gene Promoter Sequencing
Primer pair name | Genomic coordinate GRCh38 | Forward primer sequence 5′ > 3’ | Reverse primer sequence 5′ > 3’ |
|---|---|---|---|
COL5A1-F/R4 | chr9:134641407–134,641,983 | CGAAGTGATCAAACCTCGGG | GCTCGACTTTGGCCCGC |
COL5A1-F/R6 | chr9:134640893–134,641,555 | GAGAGACGCCCACCTACCAC | TTCTCTGAGAGCCGGTAGGC |
COL5A1-F/R7 | chr9:134641693–134,642,213 | CCGGGCTCTGATTTGCTG | GCTTTCCAGCGGGTATGGA |
COL5A1-F/R8 | chr9:134642371–134,643,040 | CTTCGCCCGCAGAACTTTTC | TCATCCCAACCACTCACAGC |
COL5A1-F/R9 | chr9:134642076–134,642,671 | CTAAAGTGGTGCGGTCCCTG | GTTCTGCTGTAGGGCTGTGA |