Fig. 2
From: β-blocker eye drops affect ocular surface through β2 adrenoceptor of corneal limbal stem cells
The expressions of CK3, CK14 and CK19 in the corneal limbal epithelium after levobunolol treatment. The corneal epithelium was scraped with 2.5 or 3 mm trephine and unceasingly received eye drops of saline (Ctrl) and levobunolol (Levo) for 4 times a day. At 2 or 3 days post wounding, the expressions of CK3 (A), CK14 (A), and CK19 (A) in the corneal limbal epithelium were detected by immunofluorescence staining. Moreover, the quantification of stained sections was carried out using the Image J software (B-D). Data are representative of means ± SD. *P < 0.05, **P < 0.01