Fig. 4
From: β-blocker eye drops affect ocular surface through β2 adrenoceptor of corneal limbal stem cells
Effects of the non-selective β-blocker levobunolol, the β1AR antagonist atenolol, and the β2AR antagonist ICI 118, 551 on the migration and proliferation of TKE2 cells. A Migration of TKE2 cells replenished with PBS, levobunolol, atenolol, or ICI 118, 551 for 24 h was observed. B Migration of TKE2 cells was analyzed using the Image J software. C Proliferation of TKE2 cells treated with PBS, levobunolol, atenolol, or ICI 118, 551 for 48 h was detected by CCK-8. Colony formation (D) and colony number (E) of TKE2 cells treated with PBS, levobunolol, atenolol, or ICI 118, 551 for 7 days. F Colony area of TKE2 cells treated with PBS, levobunolol, atenolol, or ICI 118, 551 for 7 days. G The colony areas were analyzed using the Echo microscope software. Data are representative of means ± SD. *P < 0.05, **P < 0.01