Fig. 2

The 34-mer and 7-mer induce apoptosis of RPMI8226 cells. a Fluorescence microscopy of annexin V-FITC/PI double staining. Treatment of RPMI8226 cells was as described in Fig. 1. Apoptosis was determined by annexin V-FITC staining (green dots) and double stained with propidium iodide (red dots). A representative of three independent experiments is shown. b The percentage of apoptotic cells was quantified by dividing the number of annexin V-positive cells by the total number of cells counted by phase-contrast light microscopy. Control peptide indicates cells were treated with the 5-mer. Histograms show means ± SD. ^aP < 0.005 versus peptide solvent-treated cells. ^bP < 0.0002 versus VEGF-treated cells. ^cP < 0.001 versus 34-mer-treated cells