Fig. 2

(A) Anterior region of the explanted cornea in the centre, PAS staining. The epithelium (upper edge of the image) is largely normally stratified, the Bowman layer is completely destroyed. Subepithelially there is a keratocyte-rich “fibrosis band” as a result of the PTK performed. Unobtrusive stroma at the lower edge of the image. Descemet’s membrane and endothelium are not depicted, but they appear normal. Magnification: x400 (B) In AMP staining for acid mucopolysaccharides, the blue deposits typical of macular corneal dystrophy are found mainly in the stroma, but also in the endothelium. Interestingly, the acidic mucopolysaccharides are found increased in the PTK induced “subepithelial fibrosis band”. Masson stain (for granular corneal dystrophy) and Congo red stain (for lattice corneal dystrophy) were negative. Magnification: x200