Table 1 Summary of proteins surveyed for and identified in the Skeie and Mahajan [5], choroid-RPE proteome analysis. The relative abundance of proteins in regions of the choroid-RPE complex is represented by the average peptide hits in the periphery, macula, and fovea. Note, this MS/MS data is provided by the Skeie and Mahajan [5], study (Key: P: Involved in progesterone signaling; A: Involved in androgen signaling; G: Involved in glucocorticoid signaling; E: Involved in estrogen signaling; M: Involved in mineralocorticoid signaling; Y: Yes, identified in dataset; N: No, not identified in dataset). Gene names and abbreviations were verified with the GeneCards database [7]
| Â | Steroid(s) | Pathway(s) | Further Pathway/Protein Information | Gene name | Protein Name | Identified in the Skeie and Mahajan [5], dataset (Y/N) | Average peptide hits in periphery | Average peptide hits in macula | Average peptide hits in fovea |
|---|---|---|---|---|---|---|---|---|---|
1 | P, A | Non-classical Progesterone Pathway | PI3K-AKT pathway | AKT2 | AKT Serine/Threonine Kinase 2 | Y | 13.66666667 | 0 | 1.333333333 |
2 | A | Classical & Non-classical Androgen Pathway | – | AR | Androgen Receptor | Y | 0 | 0 | 0.666666667 |
3 | G | Non-classical Glucocorticoid Pathway | – | EGFR | Epidermal Growth Factor Receptor | Y | 9.333333333 | 12.66666667 | 19.33333333 |
4 | G | Classical Glucocorticoid Pathway | – | FLT1 | Fms Related Receptor Tyrosine Kinase 1 | Y | 31.33333333 | 2 | 2.333333333 |
5 | P | Non-classical Progesterone Pathway | – | FYN | FYN Proto-Oncogene, Src Family Tyrosine Kinase | Y | 9.666666667 | 12 | 9.666666667 |
6 | P, A, G | Non-classical Progesterone/Androgen/Glucocorticoid Pathway | GABA Subunit | GABRA1 | Gamma-Aminobutyric Acid Type A Receptor Subunit Alpha1 | Y | 8 | 0 | 0 |
7 | P, A, G | Non-classical Progesterone/Androgen/Glucocorticoid Pathway | GABA Subunit | GABRA2 | Gamma-Aminobutyric Acid Type A Receptor Subunit Alpha2 | Y | 0 | 8.666666667 | 6.666666667 |
8 | P, A, G | Non-classical Progesterone/Androgen/Glucocorticoid Pathway | Â | GABRB2 | Gamma-Aminobutyric Acid Type A Receptor Subunit Beta2 | Y | 0 | 9.333333333 | 8.333333333 |
9 | G | Non-classical Glucocorticoid Pathway | – | GRB2 | Growth Factor Receptor Bound Protein 2 | Y | 15 | 12.33333333 | 16 |
10 | P | Non-classical Progesterone Pathway | – | GSK3B | Glycogen Synthase Kinase 3 Beta | Y | 1.333333333 | 3.333333333 | 6.333333333 |
11 | G | Non-classical Glucocorticoid Pathway | – | HRAS | HRas Proto-Oncogene, GTPase | Y | 3 | 13.33333333 | 8.666666667 |
12 | G | Classical Glucocorticoid Pathway | – | KDR | Kinase Insert Domain Receptor | Y | 4 | 3 | 0 |
13 | G | Non-classical Glucocorticoid Pathway | – | MAP2K1 | Mitogen-Activated Protein Kinase Kinase 1 | Y | 1 | 6.333333333 | 3.666666667 |
14 | P, A, G | Non-classical Progesterone/Androgen/Glucocorticoid Pathway | ERK1/2 pathway | MAPK1 | Mitogen-Activated Protein Kinase 1 | Y | 32 | 20.33333333 | 28.33333333 |
15 | P, A, G | Non-classical Progesterone/Androgen/Glucocorticoid Pathway | ERK1/2 pathway | MAPK3 | Mitogen-Activated Protein Kinase 3 | Y | 29.33333333 | 19.33333333 | 24.66666667 |
16 | P | Non-classical Progesterone Pathway | – | NENF | Neudesin Neurotrophic Factor | Y | 0 | 0 | 4.666666667 |
17 | G | Classical Glucocorticoid Pathway | GILZ signaling | NFATC2 | Nuclear Factor Of Activated T Cells 2 | Y | 0 | 0.666666667 | 1.666666667 |
18 | G | Classical Glucocorticoid Pathway | GILZ signaling | NFKB1 | Nuclear Factor Kappa B Subunit 1 | Y | 0 | 0.666666667 | 0 |
19 | G | Classical Glucocorticoid Pathway | GILZ signaling | NFKB2 | Nuclear Factor Kappa B Subunit 2 | Y | 0 | 2 | 0 |
20 | G | Classical Glucocorticoid Pathway | – | NR3C1 | Nuclear Receptor Subfamily 3 Group C Member 1 | Y | 0 | 0 | 0.666666667 |
21 | P | Non-classical Progesterone Pathway | Mediator of several pathways | PGRMC1 | Progesterone Receptor Membrane Component 1 | Y | 36 | 29.66666667 | 48.66666667 |
22 | P | Non-classical Progesterone Pathway | Binding partner for PGRMC1 | PGRMC2 | Progesterone Receptor Membrane Component 2 | Y | 0 | 15.33333333 | 17.66666667 |
23 | P | Non-classical Progesterone Pathway | PI3K-AKT pathway | PIK3CB | Phosphatidylinositol-4,5-Bisphosphate 3-Kinase Catalytic Subunit Beta | Y | 59.66666667 | 0 | 0 |
24 | P | Non-classical Progesterone Pathway | PI3K-AKT pathway | PIK3R1 | Phosphoinositide-3-Kinase Regulatory Subunit 1 | Y | 1 | 0 | 1.333333333 |
25 | P | Non-classical Progesterone Pathway | – | PRKCA | Protein Kinase C Alpha | Y | 126.6666667 | 5 | 7.666666667 |
26 | P | Non-classical Progesterone Pathway | – | PRKCB | Protein Kinase C Beta | Y | 97.66666667 | 4.333333333 | 6.333333333 |
27 | P | Non-classical Progesterone Pathway | – | PRKCD | Protein Kinase C Delta | Y | 1 | 3.333333333 | 2.333333333 |
28 | P | Non-classical Progesterone Pathway | – | PRKCI | Protein Kinase C Iota | Y | 0.666666667 | 3.666666667 | 0 |
29 | G | Non-classical Glucocorticoid Pathway | – | RAF1 | Raf-1 Proto-Oncogene, Serine/Threonine Kinase | Y | 0 | 1.666666667 | 0 |
30 | P, G | Non-classical Progesterone/Glucocorticoid Pathway | – | RELA | RELA Proto-Oncogene, NF-KB Subunit | Y | 0 | 0.666666667 | 0 |
31 | A | Non-classical Androgen Pathway | – | RPS6KA1 | Ribosomal Protein S6 Kinase A1 | Y | 4.666666667 | 6 | 3 |
32 | A | Non-classical Androgen Pathway | – | RPS6KA2 | Ribosomal Protein S6 Kinase A2 | Y | 2 | 2.333333333 | 1 |
33 | A | Non-classical Androgen Pathway | – | RPS6KA3 | Ribosomal Protein S6 Kinase A3 | Y | 4.333333333 | 3.333333333 | 4.666666667 |
34 | P | Non-classical Progesterone Pathway | Binding partner for PGRMC1 | SERBP1 | SERPINE1 MRNA Binding Protein 1 | Y | 7.666666667 | 11.33333333 | 13.33333333 |
35 | A | Non-classical Androgen Pathway | – | SHBG | Sex Hormone Binding Globulin | Y | 18 | 14.33333333 | 9.666666667 |
36 | G | Classical Glucocorticoid Pathway | GILZ signaling | SMAD2 | SMAD Family Member 2 | Y | 6.666666667 | 6.666666667 | 12.33333333 |
37 | G | Classical Glucocorticoid Pathway | GILZ signaling | SMAD3 | SMAD Family Member 3 | Y | 7.666666667 | 1 | 12.66666667 |
38 | G | Non-classical Glucocorticoid Pathway | – | SRC | SRC Proto-Oncogene, Non-Receptor Tyrosine Kinase | Y | 14 | 9.333333333 | 11.66666667 |
39 | G | Classical Glucocorticoid Pathway | GILZ signaling | TGFB1 | Transforming Growth Factor Beta 1 | Y | 1.333333333 | 1.666666667 | 1.666666667 |
41 | P, A | Non-classical Progesterone Pathway | PI3K-AKT pathway | AKT1 | AKT Serine/Threonine Kinase 1 | N | – | – | – |
42 | P, A | Non-classical Progesterone Pathway | PI3K-AKT pathway | AKT3 | AKT Serine/Threonine Kinase 3 | N | – | – | – |
43 | A | Non-classical Androgen Pathway | – | BAD | BCL2 Associated Agonist Of Cell Death | N | – | – | – |
44 | P | Classical & Non-classical Progesterone Pathways | Neurotrophic protein | BDNF | Brain Derived Neurotrophic Factor | N | – | – | – |
45 | G | Classical Glucocorticoid Pathway | GILZ signaling | CCL2 | C-C Motif Chemokine Ligand 2 | N | – | – | – |
46 | G | Classical Glucocorticoid Pathway | GILZ signaling | CCL3 | C-C Motif Chemokine Ligand 3 | N | – | – | – |
47 | P | Non-classical Progesterone Pathway | – | CX3CR1 | C-X3-C Motif Chemokine Receptor 1 | N | – | – | – |
48 | P | Non-classical Progesterone Pathway | – | CREBBP | CREB Binding Protein | N | – | – | – |
49 | P | Non-classical Progesterone Pathway | – | CYB5D2 | Cytochrome B5 Domain Containing 2 | N | – | – | – |
50 | P | Non-classical Progesterone Pathway | – | EP300 | E1A Binding Protein P300 | N | – | – | – |
51 | E | Estrogen Signaling | – | ESR1 | Estrogen Receptor 1 | N | – | – | – |
52 | E | Estrogen Signaling | – | ESR2 | Estrogen Receptor 2 | N | – | – | – |
53 | E | Estrogen Signaling | – | ESRRA | Estrogen Related Receptor Alpha | N | – | – | – |
54 | E | Estrogen Signaling | – | ESRRB | Estrogen Related Receptor Beta | N | – | – | – |
55 | E | Estrogen Signaling | – | ESRRG | Estrogen Related Receptor Gamma | N | – | – | – |
56 | P | Non-classical Progesterone Pathway | – | FGF2 | Fibroblast Growth Factor 2 | N | – | – | – |
57 | G | Classical Glucocorticoid Pathway | GILZ signaling | FOXP3 | Forkhead Box P3 | N | – | – | – |
58 | P,A,G | Estrogen Signaling | – | GPER1 | G Protein-Coupled Estrogen Receptor 1 | N | – | – | – |
59 | P,A,G | Non-classical Androgen Pathway | – | GPRC6A | G Protein-Coupled Receptor Class C Group 6 Member A | N | – | – | – |
60 | P,A,G | Non-classical Progesterone/Androgen/Glucocorticoid Pathway | GABA Subunit | GABRA3 | Gamma-Aminobutyric Acid Type A Receptor Subunit Alpha3 | N | – | – | – |
61 | P,A,G | Non-classical Progesterone/Androgen/Glucocorticoid Pathway | GABA Subunit | GABRA4 | Gamma-Aminobutyric Acid Type A Receptor Subunit Alpha4 | N | – | – | – |
62 | P,A,G | Non-classical Progesterone/Androgen/Glucocorticoid Pathway | GABA Subunit | GABRA5 | Gamma-Aminobutyric Acid Type A Receptor Subunit Alpha5 | N | – | – | – |
63 | P,A,G | Non-classical Progesterone/Androgen/Glucocorticoid Pathway | GABA Subunit | GABRA6 | Gamma-Aminobutyric Acid Type A Receptor Subunit Alpha6 | N | – | – | – |
64 | P,A,G | Non-classical Progesterone/Androgen/Glucocorticoid Pathway | GABA Subunit | GABRB1 | Gamma-Aminobutyric Acid Type A Receptor Subunit Beta1 | N | – | – | – |
65 | P,A,G | Non-classical Progesterone/Androgen/Glucocorticoid Pathway | GABA Subunit | GABRB3 | Gamma-Aminobutyric Acid Type A Receptor Subunit Beta3 | N | – | – | – |
66 | P,A,G | Non-classical Progesterone/Androgen/Glucocorticoid Pathway | GABA Subunit | GABRG1 | Gamma-Aminobutyric Acid Type A Receptor Subunit Gamma1 | N | – | – | – |
67 | P,A,G | Non-classical Progesterone/Androgen/Glucocorticoid Pathway | GABA Subunit | GABRG2 | Gamma-Aminobutyric Acid Type A Receptor Subunit Gamma2 | N | – | – | – |
68 | P,A,G | Non-classical Progesterone/Androgen/Glucocorticoid Pathway | GABA Subunit | GABRG3 | Gamma-Aminobutyric Acid Type A Receptor Subunit Gamma3 | N | – | – | – |
69 | P,A,G | Non-classical Progesterone/Androgen/Glucocorticoid Pathway | GABA Subunit | GABRR1 | Gamma-Aminobutyric Acid Type A Receptor Subunit Rho1 | N | – | – | – |
70 | E | Non-classical Androgen Pathway | – | GABRR2 | Gamma-Aminobutyric Acid Type A Receptor Subunit Rho2 | N | – | – | – |
71 | A | Non-classical Androgen Pathway | – | GABRR3 | Gamma-Aminobutyric Acid Type A Receptor Subunit Rho3 | N | – | – | – |
72 | G | Classical Glucocorticoid Pathway | GILZ signaling | IL1R1 | Interleukin 1 Receptor Type 1 | N | – | – | – |
73 | G | Classical Glucocorticoid Pathway | GILZ signaling | IL17RC | Interleukin 17 Receptor C | N | – | – | – |
74 | G | Classical Glucocorticoid Pathway | GILZ signaling | JUN | Jun Proto-Oncogene, AP-1 Transcription Factor Subunit | N | – | – | – |
75 | P | Non-classical Progesterone Pathway | – | KEAP1 | Kelch Like ECH Associated Protein 1 | N | – | – | – |
76 | P | Non-classical Progesterone Pathway | – | MAFF | MAF BZIP Transcription Factor F | N | – | – | – |
77 | P | Non-classical Progesterone Pathway | – | MAFG | MAF BZIP Transcription Factor G | N | – | – | – |
78 | P | Non-classical Progesterone Pathway | – | MAFK | MAF BZIP Transcription Factor K | N | – | – | – |
79 | P | Non-classical Progesterone Pathway | Involved in PGRMC1-mediated BDNF release | MAPK7 | Mitogen-Activated Protein Kinase 7 | N | – | – | – |
80 | G | Classical Glucocorticoid Pathway | GILZ signaling | NFATC1 | Nuclear Factor Of Activated T Cells 1 | N | – | – | – |
81 | G | Classical Glucocorticoid Pathway | GILZ signaling | NFATC3 | Nuclear Factor Of Activated T Cells 3 | N | – | – | – |
82 | G | Classical Glucocorticoid Pathway | GILZ signaling | NFATC4 | Nuclear Factor Of Activated T Cells 4 | N | – | – | – |
83 | G | Classical Glucocorticoid Pathway | GILZ signaling | NFAT5 | Nuclear Factor Of Activated T Cells 5 | N | – | – | – |
84 | P | Non-classical Progesterone Pathway | – | NFE2L2 | Nuclear Factor, Erythroid 2 Like 2 | N | – | – | – |
85 | M | Classical Mineralocorticoid Pathway | – | NR3C2 | Nuclear Receptor Subfamily 3 Group C Member 2 | N | – | – | – |
86 | P | Non-classical Progesterone Pathway | – | PIK3CA | Phosphatidylinositol-4,5-Bisphosphate 3-Kinase Catalytic Subunit Alpha | N | – | – | – |
87 | P | Non-classical Progesterone Pathway | – | PIK3CD | Phosphatidylinositol-4,5-Bisphosphate 3-Kinase Catalytic Subunit Delta | N | – | – | – |
88 | P | Non-classical Progesterone Pathway | – | PIK3CG | Phosphatidylinositol-4,5-Bisphosphate 3-Kinase Catalytic Subunit Gamma | N | – | – | – |
89 | P | Non-classical Progesterone Pathway | – | PGR | Progesterone Receptor | N | – | – | – |
90 | P | Non-classical Progesterone Pathway | – | PAQR5 | Progestin And AdipoQ Receptor Family Member 5 | N | – | – | – |
91 | P | Classical Progesterone Pathway | – | PAQR6 | Progestin And AdipoQ Receptor Family Member 6 | N | – | – | – |
92 | P | Non-classical Progesterone Pathway | PI3K-AKT pathway | PAQR7 | Progestin And AdipoQ Receptor Family Member 7 | N | – | – | – |
93 | P | Non-classical Progesterone Pathway | PI3K-AKT pathway | PAQR8 | Progestin And AdipoQ Receptor Family Member 8 | N | – | – | – |
94 | P | Non-classical Progesterone Pathway | – | PAQR9 | Progestin And AdipoQ Receptor Family Member 9 | N | – | – | – |
95 | G | Classical Glucocorticoid Pathway | GILZ signaling | PRKCZ | Protein Kinase C Zeta | N | – | – | – |
96 | G | Classical Glucocorticoid Pathway | GILZ signaling | REL | REL Proto-Oncogene, NF-KB Subunit | N | – | – | – |
97 | G | Classical Glucocorticoid Pathway | GILZ signaling | RELB | RELB Proto-Oncogene, NF-KB Subunit | N | – | – | – |
98 | G | Classical Glucocorticoid Pathway | – | SMAD4 | SMAD Family Member 4 | N | – | – | – |
99 | P | Non-classical Progesterone Pathway | – | TSC22D3 | TSC22 Domain Family Member 3 (GILZ) | N | – | – | – |
100 | P | Non-classical Progesterone Pathway | PI3K-AKT pathway | VEGFA | Vascular Endothelial Growth Factor A | N | – | – | – |
101 | A | Non-classical Androgen Pathway | Membrane androgen receptor | SLC39A9 | Solute Carrier Family 39 Member 9 | N | – | – | – |