Fig. 1

In vitro study. A A CCK8 assay was used to assess the proliferation of HUVECs stimulated with Scg3 antibodies for 12, 24 and 48 h. After 24 h, the proliferation of HUVECs stimulated by Scg3 antibodies was significantly lower than that of the other 2 groups. B In the tube formation assay, after 7 h, Scg3 antibodies (1 µg/ml Scg3 and 2 µg/ml anti-Scg3) significantly inhibited the angiogenesis of vascular endothelial cells compared with the Scg3 and control treatments alone. C In the wound healing migration assay, HUVECs were cultured in different media. The number of cells that migrated into the denuded area was quantified. After statistical analysis by one-way ANOVA, the cell proliferation in the Scg3 antibody mixture group was significantly lower than that in the Scg3 group, and there was no significant difference between the Scg3 group and the control group. D Representative images of HUVECs before and after migration. (*P < 0.05)