Association between diabetic retinopathy in type 2 diabetes and the ICAM-1 rs5498 polymorphism: a meta-analysis of case-control studies

Background Genetic studies have reported contradictory results on the association between the intercellular adhesion molecule-1 (ICAM-1) rs5498 polymorphism and diabetic retinopathy (DR) risk in type 2 diabetic patients. We aimed to perform a systematic literature search and conduct random-effects meta-analysis to provide a quantitative evaluation. Methods We searched Pubmed, Embase, Scopus, Web of Science and Wanfang databases from inception up to January 2018. Allelic and genotype frequencies of rs5498 was compared between DR cases and controls. Odds ratios (OR) and 95% confidence intervals (CI) were calculated using a random effects model. Results Nine studies involving a total of 1792 cases and 1400 controls met our inclusion criteria. We did not find any significant association between rs5498 and DR risk at the dominant model (GG + GA versus AA, OR = 1.00, 95% CI: 0.66–1.50, P = 0.987), the recessive model (GG versus GA + AA, OR = 1.24, 95% CI: 0.86–1.77, P = 0.245), the GG versus AA contrast (OR = 1.14, 95% CI: 0.68–1.92, P = 0.611), and the G allele versus A allele contrast (OR = 1.08, 95% CI: 0.81–1.45, P = 0.592). Subgroup analysis by ethnicity showed no association in Asian populations (G allele versus A allele: OR = 1.05, 95% CI: 0.76–1.44, P = 0.790). Subgroup analysis by DR subtype also did not reveal any association of rs5498 with proliferative DR (G allele versus A allele: OR = 1.34, 95% CI: 0.71–2.52, P = 0.364) and non-proliferative DR (G allele versus A allele: OR = 0.71, 95% CI: 0.43–1.17, P = 0.180). Conclusion Our meta-analyses provide no evidence of the association of rs5498 with DR in type 2 diabetic patients. Electronic supplementary material The online version of this article (10.1186/s12886-018-0961-5) contains supplementary material, which is available to authorized users.


Background
Diabetic retinopathy (DR) is the single most common complication of diabetes mellitus and the leading cause of blindness in working-aged adults worldwide [1]. Despite numerous breakthroughs in the development of novel pharmacological agents for DR in the last decade, the incidence of DR remains high and 90% of type 1 and 60% of type 2 diabetes patients suffer from the disease. Body mass index (BMI), increased duration of diabetes, ineffective blood glucose control, and ineffective blood pressure control are the major risk factors for DR [1,2]. However, they do not adequately predict disease progression in individual patients, suggesting the presence of a genetic component. Identification of the specific genetic risk factors for DR susceptibility is an area of substantial research and could unravel druggable targets for the purpose of treatment or even prevention.
In recent years, emerging evidence has highlighted the potential role of intercellular adhesion molecule-1 (ICAM-1) in the development of DR. ICAM-1 is a immunoglobulin-(Ig)-like transmembrane glycoprotein expressed on the surface of leukocytes, endothelial cells, and epithelial cells [3]. It influences the adhesion of circulating immune cells to the endothelium and contributes to immune cell migration and perivascular infiltration. Increased levels of ICAM-1 and its ligands have been observed in patients with DR and retina of animal models [4][5][6][7]. ICAM-1 blockade with monoclonal antibodies effectively prevents diabetic retinal leukostasis, vascular leakage, and capillary nonperfusion in experimental DR [4]. Similarly, when the bioactivity of the ICAM-1 counter receptor CD18 is inhibited, diabetic retinal leukocyte adhesion is potently suppressed [5].
Because ICAM-1 has been implicated in DR development, multiple studies have investigated how genetic variation at ICAM-1 is related to DR risk. More than 100 single-nucleotide polymorphisms (SNPs) were identified in the ICAM-1 gene. The best studied SNP is a G/A polymorphism in exon6 at codon 469 (rs5498), resulting in a lysine (Lys) to glutamine (Glu) substitution in Ig-like domain 5 that is essential for dimerisation, surface presentation and solubilisation of the protein [8]. This polymorphism has been shown to influence the interaction of ICAM-1 with leukocyte function-associated antigen-1 (LFA-1) and the macrophage-1 antigen during leukocyte adhesion [8]. In the present study, we aimed to provide a quantitative evaluation of the association between DR in type 2 diabetes and the ICAM-1 rs5498 polymorphism.

Literature search
The search strategy for this meta-analysis was comprehensive, aiming to retrieve the largest possible number of relevant studies. We systematically screened 5 electronic databases including Pubmed (Additional file 1), Embase, Scopus, Web of Science and Wanfang for articles published between January 1990 and January 2018. The following keywords were used: intercellular adhesion molecule-1, K469E, rs5498, diabetic retinopathy, type 2 diabetes, and polymorphism. In addition, the reference lists of all the retrieved papers and relevant reviews were manually searched for eligible papers. We only included published studies with full-text articles available. In case of overlap between articles reporting on the same cohort, we included the study with the largest cohort. Our meta-analysis adhered to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) Statement (Additional file 2) [9].

Inclusion and exclusion criteria
One reviewer performed the initial screen of all papers identified by the electronic searches. Studies were excluded when the title clearly indicated that it did not meet the inclusion criteria. Where a title/abstract could not be rejected with certainty, the full text of the publications was obtained for assessment. Studies were considered eligible if they met the following criteria: 1) evaluated the frequency of the ICAM-1 rs5498 polymorphism in relation to the number of retinopathy cases and controls; 2) published in English or Chinese; and 3) published prior to January 2018 unless an online version of the study had been released prior to this date; and 4) reported odds ratios (ORs) and 95% confidence intervals (95% CIs) or data to calculate them. Case-only and case series studies with no control population were excluded, as well as studies based only on phenotypic tests, reviews, meta-analysis. We also excluded unpublished studies or gray literature because we expected them to contain insufficient reporting for our analysis.

Data extraction and quality assessment
Data extraction was performed by the first author and entered into predesigned electronic tables. The second author checked the extracted data. Disagreements were resolved by discussion between the two authors. The following items were considered: first author, year of publication, location of the study, ethnicity, number of cases and controls, diagnostic criteria, allele or genotype frequency, Hardy-Weinberg equilibrium (HWE) status, and genotyping method. The methodological quality of each study was assessed by the Newcastle-Ottawa Scale, which was used for its simplicity in comparing observational studies. Studies were evaluated based on cohort selection, comparability and ascertainment of exposure using nine multiple-choice questions. Studies were deemed of low quality if the total score was 5 or lower [10].

Statistical analyses
Statistical analysis was performed using STATA 11 (Sta-taCorp, College Station, TX). To assess HWE status, we used a publicly available program (http://ihg.gsf.de/cgi-b in/hw/hwa1.pl). For our main analysis, we compared allele frequencies (the -encoding allele G versus the -encoding allele A) between cases and controls. We also evaluated a dominant model (GG + AG versus AA) and a recessive model (GG versus AG + AA) for the G allele. All associations were presented as ORs with their corresponding 95% CIs. Heterogeneity was evaluated by Cochran's Q and the I 2 statistic. When heterogeneity was high (I 2 > 50%, P < 0.10), a pooled analysis was conducted using DerSimonian and Laird random effects models [11]. The significance of the summary OR was determined using an asymptotic Z-test. We evaluated publication bias or selective reporting by using funnel plots as well as Egger's regression intercept test.

Heterogeneity and publication bias
The present meta-analysis revealed heterogeneity among the included studies (I 2 ranged from 65.3-84.0%). The funnel plot did not demonstrate apparent asymmetry (Fig. 5). Egger's test also did not indicate any evidence of publication bias (Table 3).

Discussion
DR is the most frequent microvascular complication from type 2 diabetes. A large body of clinical and experimental literature has indicates that leucocyte adhesion to the retinal vasculature plays an important role in the pathogenesis of DR. As a central mediator of leukocyte adhesion to and transmigration across the endothelium,  DR diabetic retinopathy, HWE Hardy-Weinberg equilibrium, MAF minor allele frequency, NA not available, NOS Newcastle-Ottawa scale, NPDR non-proliferative diabetic retinopathy, PCR-LDR polymerase chain reactionligase detection reaction, PCR-RFLP polymerase chain reaction-restriction fragment length polymorphism, PDR proliferative diabetic retinopathy the gene encoding ICAM-1 is thought to be involved in the development of DR. The current mete-analytic review was conducted to verify the genetic contribution of a common SNP, rs5498 in the ICAM-1 gene to retinopathy risk in type 2 diabetes. Our results showed a lack of association between the ICAM-1 rs5498 polymorphism and risk of retinopathy in type 2 diabetes. Subgroup analysis by ethnicity did not reveal any significant association in Asian populations. In addition, when DR were subdivided into two main stages: non-proliferative and proliferative, we found no association of the ICAM-1 rs5498 polymorphism with the risk of non-proliferative and proliferative DR.
The ICAM-1 rs5498 polymorphism results in substitution of an A with a G nucleotide and replaces lysine (K) with a glutamic acid (E). It is thought that the SNP affects mRNA splicing patterns that modify cell-cell interactions and influence inflammatory response [8]. Kamiuchi et al. initially reported a positive association between rs5498 genotypes and retinopathy in type 2 diabetes, using a very small sample size (81 cases and 50 controls) [12]. However, their findings were not replicated by all other studies on the topic. It was noteworthy that the study by Balasubbu et al. with 704 participants and the study by Lv et al. involving 782 participants were the two having relatively large sample sizes among the included studies, but they did not identify any statistically significant association of rs5498 with DR [17,19]. We could not exclude the possibility that false-positive findings may be obtained from studies with small sample sizes like one conducted by Kamiuchi et al. Combining published data from nine studies involving 3192 participants, this is the largest meta-analysis on the relationship between the ICAM-1 rs5498 polymorphism and retinopathy in type 2 diabetes. Previous meta-analyses on the same topic included fewer studies (Su et [21][22][23]. Results from this meta-analysis differed from those of the meta-analysis by Su et al., which found an association between rs5498 and DR in type 2 diabetes. This was probably because in addition to the five studies included by Su et al. [21], we included four recently published case-control studies and conducted the pooled analyses with a larger sample size [22,23]. Sun     rs5498 with DR; their results were consistent with our calculations. Compared to the previous meta-analyses, our study had several strengths. First, considering that the cause and development of type 1 diabetes and type 2 diabetes were different, we only included retinopathy subjects of type 2 diabetes as cases in our analyses. We did not take into account the results from type 1 diabetes. Second, in addition to subgroup analyses by ethnicity, we performed subtype-specific analyses to evaluate the relation of rs5498 with the risk of non-proliferative and proliferative DR, respectively. Such evaluations were not performed by the previous meta-analyses. Third, we performed influential analysis to ensure the robustness of our combined estimations. Since most of the included studies were conducted on Asian populations (n = 8), it became evident from this meta-analysis that further studies should include larger  [14]; their findings should be replicated in other Caucasian populations, including British, German, and French populations. Besides rs5498, more than 200 polymorphisms have been identified in the ICAM-1 gene. We could not exclude the possibility that other ICAM-1 polymorphisms played a role in the susceptibility to type 2 DR. The study by Simões et al. identified a significant association between the ICAM-1 rs1801714 polymorphsim and non-proliferative DR in type 2 diabetes [24]. In addition, a Chinese study assessed the association of DR with the rs1799969 polymorphism which was located in exon 4 of the ICAM-1 gene [25]. Because the evidence base for other ICAM-1polymorphisms was very small at the time of planning our meta-analysis and consequently we chose to focus our analysis only on the rs5498 polymorphism. It would be advisable that in the future attention should be paid to the relationship between other ICAM-1 polymorphisms and DR in type 2 diabetes.
Several limitations should be considered. First, this study was limited by the unavailability of individual patient data that would allow the identification of potential interactions of rs5498 with specific disease characteristics, including glycemic control, blood pressure control, and hyperlipemia. These factors might have contributed to the lack of success in identifying positive results in association studies for rs5498. Second, like all meta-analyses, the present study was susceptible to reporting biases. Despite the use of comprehensive strategies to identify eligible studies for rs5498, we could not exclude the possibility that some studies might have been erroneously excluded. Third, we did not evaluate the relation of serum soluble ICAM-1 (sICAM-1) levels with DR because of limited published data. There was some evidence suggesting that circulating sICAM levels were positively associated with DR prevalence in type 2 diabetes [14,26]. It is recommended that future association studies should perform sICAM-1 measurement and evaluate the effects of rs5498 on sICAM levels in DR patients, which could help clarify the role of rs5498 in DR development.
In conclusion, Our meta-analysis has shown that there is no significant association of the ICAM-1 rs5498 polymorphism with DR in type 2 diabetes. Further investigation involving non-Asian populations is warranted on the association between this polymorphism and DR, particularly studies with larger sample size that adjust for confounding variables.