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Table 2 Characterization of some very likely endogenous cytokeratins.

From: Attempting to distinguish between endogenous and contaminating cytokeratins in a corneal proteomic study

CKs

Spot number in gels (6%=',12%=*)

UniProtKB/Swiss-Prot

Observed M (kDA)

Theoretical M (kDA)

Observed pI

Theoretical pI

Coverage (%)

Fraction of protein covered (%)1

3

6503'

K2C3_HUMAN

64

64.5

6.90

6.12

12

56

 

5213*

P12035

28

 

6.60

 

3

34

 

5610*

 

60

 

7.00

 

31

88

 

5713*

 

80

 

7.05

 

9

56

4

8402'

K2C4_HUMAN

62

57.3

7.80

6.25

6

21

 

5602'

P19013

65

 

6.70

 

4

57

 

5610*

 

60

 

7.00

 

3

24

12

0201'

K1C12_HUMAN Q99456

51

53,4

4.30

4.70

9

40

13

0301'

K1C13_HUMAN

56

49.6

4.40

4.91

25

71

 

1205'

P13646

56

 

4.80

 

35

71

 

1617*

 

50

 

5.05

 

16

64

 

1618*

 

50

 

5.10

 

34

90

 

2402*

 

38

 

5.50

 

5

19

15

2201'

K1C15_HUMAN P19012

52

49.2

5.60

4.71

16

78

19

2513*

K1C19_HUMAN

39

44.1

5.40

5.04

23

81

 

1517*

P08727

40

 

5.30

 

17

81

  1. 1This was determined by calculating the fraction of the protein covered from the first amino acid in the most N-terminal peptide to the last amino acid in the most C-terminal peptide observed.