Figure 1
Schematic representation of the isolated perfused vessel system. The isolated pig retinal arteriole is cannulated at both ends and perfused intraluminally whilst maintained in a temperature controlled bath on the stage of an inverted microscope. VEGF can be administered intraluminally via an HPLC valve. ET-1 and bevacizumab are delivered extraluminally by addition to the bathing solution. Vessel diameter is monitored every two seconds by an automated frame grabbing and vessel diameter measurement routine.