Figure 3

Retinal thickness (A and B) and number of cells in the ganglion cell layer (GCL) of diabetic and control rat retinas (C and D). (A) The thickness of the entire retina of 4 eyes from each group was measured 10 sections in the posterior retina 100 to 500 μm from the optic disc. Image analysis measurements were taken using a Nikon DXM 1200 digital camera (Tokyo, Japan) mounted onto an Eclipse Nikon E-800 microscope for image acquisition. Barr 50 μm. (B) Statistical analysis was carried out using one-way analysis of variance and Newman-Keuls Multiple Comparison Post-Test to compare diabetic groups and controls. All elements out of the two standard deviations were eliminated. (**p <0.001, ***p <0.0001). (C) Number of cells in the GCL of diabetic and control rat retinas. The GCL was analyzed in H&E-stained cryosections of diabetic (37 weeks after onset of diabetes) and age-matched control retinas. Samples were evaluated in a masked fashion. Control animals had retinal GCLs with densely packed cells. There was a typically uniform distribution of cells, except for an occasional blood vessel that made a little space between cells (arrows) Barr 50 μm total frame 275 μm. (D) Statistical analysis was carried out using one-way analysis of variance and Newman-Keuls Multiple Comparison Post-Test to compare the number of ganglion cells between the groups. All elements out of the two standard deviations were eliminated. (***p <0.0001).