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Figure 6 | BMC Ophthalmology

Figure 6

From: Oxidative stress causes ERK phosphorylation and cell death in cultured retinal pigment epithelium: Prevention of cell death by AG126 and 15-deoxy-delta 12, 14-PGJ2

Figure 6

Activation of ERK by H2O2 treatment. ARPE-19 were treated with 1.5 mM H2O2 for one hour, then processed for immunohistochemical staining using anti-phospho-ERK antibody. Fig. 6A: untreated cells. Scale bar: 50 μm. Fig. 6B: H2O2 treated cells. Fig. 6C: ARPE-19 were treated with various concentrations (0.5, 1, 1.5, 2 mM) of H2O2 for 30 minutes, and then processed for Western blot analysis. Antibodies against ERK and phospho-ERK were used in this study. P: positive controls. This is a representative result from 3 similar experiments.

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BMC Ophthalmology

ISSN: 1471-2415

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