Fig. 4
GPX4 may a target of lncRNA ENST00000586817 by cis mechanism and inhibit LECs ferroptosis in ARC. (A) MeRIP-Seq analysis on the distribution of m6A peak within lncRNA ENST00000586817. (B) Diagram showing ENST00000586817 and GPX4 locus on chromosome 19. (C) qRT-PCR analysis for validation the expression of DE-lncRNA ENST00000586817 in human LECs (n = 10 each). qRT-PCR data were normalized against the GAPDH level. (D) TEM analysis indicated that mitochondria were increased in ARC (n = 3 each). (E) qRT-PCR analysis was performed on the GPX4 in human LECs (n = 10 each). qRT-PCR data were normalized against the GAPDH level. (F) Western blot analysis for the expression of GPX4 protein (n = 3 each). Significance value: ns, no significance, *p < 0.05, ****p < 0.0001