Fig. 3

Medrysone induced M2-like polarization by IL4-STAT6 pathway. A Medrysone increased the phosphorylation of STAT6. Raw264.7 was treated with or without Medrysone (5 μM) and IL-4 (10 ng/mL) for 24 h, and the expression of p-STAT6 was detected by Western Blotting. The images were quantified using Image J grayscale analysis and represented as relative pSTAT6 to STAT6. B and C The up-regulation of CD206 expression induced by Medrysone was abolished by the absence of STAT6. Raw264.7 was treated with Medysone (5 μM) for 24 h after transfection of NC or siSTAT6, and the expression of CD206 was detected by flow cytometry. The images were quantified using Image J grayscale analysis and represented as relative STAT6 to GAPDH. The data are presented as the mean ± SD of triplicate experiments. Statistical significance was determined by one-way ANOVA with Dunnett’s post hoc test. #, p < 0.05