Association analysis of cigarette smoking with onset of primary open-angle glaucoma and glaucoma-related biometric parameters
© Wang et al.; licensee BioMed Central Ltd. 2012
Received: 26 November 2011
Accepted: 14 November 2012
Published: 27 November 2012
To date, studies on the role played by cigarette smoking in primary open-angle glaucoma (POAG) remains controversial. The current study evaluated cigarette smoking as a risk factor of POAG and its relationships with vertical cup-to-disc ratio (VCDR), central corneal thickness (CCT) and intraocular pressure (IOP) in a Chinese cohort.
In a total of 248 unrelated individuals including 30 juvenile-onset POAG (JOAG), 92 adult-onset POAG (AOAG) and 126 sex-matched senile cataract controls, underwent comprehensive ophthalmic examination. Their smoking was obtained and documented by questionnaire. Association of cigarette smoking with POAG was performed using logistic regression controlled for age and sex. Effects of cigarette smoking on VCDR, IOP and CCT were analyzed with multiple linear regression.
In either JOAG or AOAG, no association of cigarette smoking was found with disease onset (P = 0.692 and 0.925 respectively). In controls and JOAG, no significant effects of smoking were found on VCDR, IOP or CCT (all P > 0.05). Smoking was found to be correlated with decreased CCT in AOAG and combined POAG (JOAG + AOAG) (P = 0.009 and 0.003), but no association with VCDR or IOP was observed (P > 0.05).
Although cigarette smoking was not found to be risk factor for onset of POAG, it was correlated with CCT in AOAG, and thus might still play a role in the disease course, especially for AOAG.
KeywordsPrimary open angle glaucoma Cigarette smoking Central corneal thickness Vertical cup-to-disc ratio Intraocular pressure
Primary open-angle glaucoma (POAG) is a group of disorders characterized by loss of retinal ganglion cells associated with optic nerve degeneration and visual field loss. It’s the second leading cause of visual loss and blindness worldwide, and affected 60 million people . POAG is associated with many clinical features, including vertical cup-to-disc ratio (VCDR), central corneal thickness (CCT) and intraocular pressure (IOP). According to age of disease onset, there are two categories of POAG: adult-onset POAG (AOAG, disease onset after 40 years) and juvenile-onset POAG (JOAG, disease onset between 3 and 40 years) . Reported risk factors of POAG include cigarette smoking, hypertension, diabetes, and family history [3–5]. Although existing studies have reported the association between cigarette smoking and POAG [6, 7], the role of cigarette smoking as a risk factor of POAG remains controversial [8, 9]. And most of these studies focused on AOAG. Especially, there are limited data of these studies in the Chinese population. In this study we investigated the association of cigarette smoking with POAG, and its relationship with VCDR, CCT and IOP in a Southern Chinese cohort.
Patient recruitment and clinical information
Demographic features of the study subjects
The diagnosis of POAG was based on the following inclusion criteria: (1) gonioscopically open anterior chamber angle, Shaffer grade III or IV; (2) characteristic optic disc damage and/or typical visual field loss; (3) highest IOP > 21 mmHg; (4) exclusion of secondary causes, e.g., trauma, uveitis, steroid-induced or exfoliation glaucoma. Juvenile-onset POAG (JOAG) was recruited based on age of disease onset between 3 and 40 years, and adult-onset POAG (AOAG) based on disease onset after 40 years. The control subjects were recruited from senile cataract surgical inpatients aged 50 and older without family history of glaucoma. All of the controls have IOP < 21 mmHg, VCDR < 0.5, and no sign of visual field loss. Eyes met the following criteria were excluded: any history or symptom of keratopathy, Marfan’s syndrome, ocular trauma, ocular surgery in prior to the recruitment, macular epiretinal membrane, macular edema, macular hemorrhage, retinal detachment, or severe cataract affecting the results of examination.
This study was approved by the Ethics Committee of Joint Shantou International Eye Center and was conducted in accordance with the Declaration of Helsinki. Written consent was obtained from each participating subject after explanation of the nature of the study.
Association and interaction analysis was performed using multiple regression implemented by the R statistical Language version 2.15.0. Association between cigarette smoking and disease was assessed in both JOAG and AOAG by logistic regression controlled age and sex. Multiple linear regression was used to analyze effects of cigarette smoking on VCDR, IOP and CCT. Two-factor interaction between smoking and age/sex was evaluated in both logistic and linear regression.
Patients and clinical data
Their clinical features were shown in Table 1. Among POAG patients, 30 were diagnosed as juvenile-onset POAG (JOAG), 92 as adult-onset POAG (AOAG). Ocular hypertension and signs of optic disc neuropathy were found in these POAG patients, but were absent in all 126 senile cataract controls.
Relationship between cigarette smoking and disease onset of POAG
Association of cigarette smoking with JOAG and AOAG in the current cohort
4.33 (0.12-5.84 × 105)
Correlation between cigarette smoking and glaucoma-related biometric parameters
Multiple linear regression analysis of effects of smoking, age and female sex on vertical cup-to-disc ratio
JOAG + AOAG
Multiple linear regression analysis of effects of smoking, age and female sex on intraocular pressure
JOAG + AOAG
Multiple linear regression analysis of effects of smoking, age and female sex on central corneal thickness
JOAG + AOAG
According to World Health Organization data, smoking has become a serious global public health problem. Among the approximately 1.3 billion smokers in the world, over 6 million die annually due to tobacco exposure, especially in . Cigarette smoking is related to many eye diseases such as cataract, age-related macular degeneration [11–13]. Existing studies on the relationship between cigarette smoking and POAG remain controversial. Findings vary among ethnic groups and study design. And most of these studies focused on AOAG. There are limited data of these studies in the Chinese population. Recently in a cohort of African-American women, Wise L. A. et al. reported that smoking might be associated with increased risk of early-onset POAG . Kang, J. H. et al. also reported cigarette smoking conferring risk to POAG . Similar findings were also reported in other independent studies [16–18]. But some other studies reported no association between smoking and POAG. In a prospective follow-up study from 1980 and 1986, respectively, to 1996, the results showed neither current smokers nor ex-smokers were at greater risk for POAG than those who had never smoked, and heavier smoking did not increase the risk of POAG . And in a systematic review, Richard et al. also reported that there was little evidence for the association between smoking and POAG . Other independent studies also reported lack of such association [9, 20]. In our Chinese cohort, smoking was not found to confer risk to disease onset in either JOAG or AOAG. Our study thus did not support cigarette smoking as a risk factor of POAG onset.
Apart from disease onset, it remains to be elucidated whether cigarette smoking is related to IOP, CCT and VCDR, which are glaucoma risk factors. In 2003, Yoshida M. et al. reported that cigarette smoking had a significantly positive association with the IOP in Japanese male individuals . Lee A. J. et al. also reported similar association . In the current study, although no significant association with IOP was observed, we found evidence that smoking could be correlated with decreased CCT in AOAG. As reported by previous studies, in individuals with thinner cornea, their IOP tends to be lower estimated [23–25]. In AOAG smokers, their IOP could possibly be lower estimated due to thinner CCT. In addition, the change of CCT in POAG may affect the disease course. A previous study on corneal thickness and functional damage in patients with ocular hypertension showed that patients with ocular hypertension plus thinner cornea had a greater risk of developing functional damage over time .
The exact reason for decreased corneal thickness in POAG smokers remains unclear. However, cigarette smoking may exert this effect through hypoxia and collagen in the cornea. Smoking has been reported to decrease oxygen and collagen production in tissues during wound healing [27, 28]. Ocular hypertension causes damage to the cornea . Smoking probably deteriorates ocular hypoxia caused by ocular hypertension , and consequently affects the biosynthesis of collagen and extracellular matrix turnover , which could be an explanation to the decreased corneal thickness.
In the current study, cigarette smoking was not found to be associated with disease onset of POAG. However, the association of smoking with decreased CCT in POAG suggested that more attention should be paid on CCT in the early stage of POAG in smokers to estimate more correct target IOP in order to better reduce the early lesion of optic nerve. Current findings thus warranted further study.
In this study, although cigarette smoking was not found to be a risk factor for onset of POAG, it was correlated with CCT in AOAG, and thus might still play a role in the disease course, especially for AOAG.
This study was supported in part by Research Grants 81000397 from the National Natural Science Foundation of China; 8151503102000019 from the Natural Science Foundation of Guangdong Province, China; 2010B031600130 from the Science and Technology Planning Project of Guangdong Province, China; and 10–020, 10–021, and 10–022 from the Joint Shantou International Eye Center, Shantou University/The Chinese University of Hong Kong.
- Quigley HA, Broman AT: The number of people with glaucoma worldwide in 2010 and 2020. Br J Ophthalmol. 2006, 90 (3): 262-267. 10.1136/bjo.2005.081224.PubMed CentralView ArticlePubMed
- Rao KN, Nagireddy S, Chakrabarti S: Complex genetic mechanisms in glaucoma: an overview. Indian J Ophthalmol. 2011, 59 (Suppl): S31-S42.PubMed CentralPubMed
- Fan BJ, Leung YF, Wang N, Lam SC, Liu Y, Tam OS, Pang CP: Genetic and environmental risk factors for primary open-angle glaucoma. Chin Med J (Engl). 2004, 117 (5): 706-710.
- Raymond V: Molecular genetics of the glaucomas: mapping of the first five “GLC” loci. Am J Hum Genet. 1997, 60 (2): 272-277.PubMed CentralPubMed
- Leske MC: The epidemiology of open-angle glaucoma: a review. Am J Epidemiol. 1983, 118 (2): 166-191.PubMed
- Cheng AC, Pang CP, Leung AT, Chua JK, Fan DS, Lam DS: The association between cigarette smoking and ocular diseases. Hong Kong Med J. 2000, 6 (2): 195-202.PubMed
- Solberg Y, Rosner M, Belkin M: The association between cigarette smoking and ocular diseases. Surv Ophthalmol. 1998, 42 (6): 535-547. 10.1016/S0039-6257(98)00002-2.View ArticlePubMed
- Kang JH, Pasquale LR, Rosner BA, Willett WC, Egan KM, Faberowski N, Hankinson SE: Prospective study of cigarette smoking and the risk of primary open-angle glaucoma. Arch Ophthalmol. 2003, 121 (12): 1762-1768. 10.1001/archopht.121.12.1762.View ArticlePubMed
- Klein BE, Klein R, Ritter LL: Relationship of drinking alcohol and smoking to prevalence of open-angle glaucoma. The Beaver Dam Eye Study. Ophthalmology. 1993, 100 (11): 1609-1613.View ArticlePubMed
- Wipfli H, Samet JM: Global economic and health benefits of tobacco control: part 1. Clin Pharmacol Ther. 2009, 86 (3): 263-271. 10.1038/clpt.2009.93.View ArticlePubMed
- Thornton J, Edwards R, Mitchell P, Harrison RA, Buchan I, Kelly SP: Smoking and age-related macular degeneration: a review of association. Eye (Lond). 2005, 19 (9): 935-944. 10.1038/sj.eye.6701978.View Article
- Lois N, Abdelkader E, Reglitz K, Garden C, Ayres JG: Environmental tobacco smoke exposure and eye disease. Br J Ophthalmol. 2008, 92 (10): 1304-1310. 10.1136/bjo.2008.141168.View ArticlePubMed
- Cong R, Zhou B, Sun Q, Gu H, Tang N, Wang B: Smoking and the risk of age-related macular degeneration: a meta-analysis. Ann Epidemiol. 2008, 18 (8): 647-656. 10.1016/j.annepidem.2008.04.002.View ArticlePubMed
- Wise LA, Rosenberg L, Radin RG, Mattox C, Yang EB, Palmer JR, Seddon JM: A prospective study of diabetes, lifestyle factors, and glaucoma among African-American women. Ann Epidemiol. 2011, 21 (6): 430-439. 10.1016/j.annepidem.2011.03.006.PubMed CentralView ArticlePubMed
- Kang JH, Wiggs JL, Rosner BA, Haines J, Abdrabou W, Pasquale LR: Endothelial nitric oxide synthase gene variants and primary open-angle glaucoma: interactions with hypertension, alcohol intake, and cigarette smoking. Arch Ophthalmol. 2011, 129 (6): 773-780. 10.1001/archophthalmol.2011.118.PubMed CentralView ArticlePubMed
- Mehra KS, Roy PN, Khare BB: Tobacco smoking and glaucoma. Ann Ophthalmol. 1976, 8 (4): 462-464.PubMed
- Wilson MR, Hertzmark E, Walker AM, Childs-Shaw K, Epstein DL: A case–control study of risk factors in open angle glaucoma. Arch Ophthalmol. 1987, 105 (8): 1066-1071. 10.1001/archopht.1987.01060080068030.View ArticlePubMed
- Bonovas S, Filioussi K, Tsantes A, Peponis V: Epidemiological association between cigarette smoking and primary open-angle glaucoma: a meta-analysis. Public Health. 2004, 118 (4): 256-261. 10.1016/j.puhe.2003.09.009.View ArticlePubMed
- Edwards R, Thornton J, Ajit R, Harrison RA, Kelly SP: Cigarette smoking and primary open angle glaucoma: a systematic review. J Glaucoma. 2008, 17 (7): 558-566. 10.1097/IJG.0b013e31815f530c.View ArticlePubMed
- Ponte F, Giuffre G, Giammanco R, Dardanoni G: Risk factors of ocular hypertension and glaucoma. The casteldaccia Eye study. Doc Ophthalmol. 1994, 85 (3): 203-210. 10.1007/BF01664928.View ArticlePubMed
- Yoshida M, Ishikawa M, Kokaze A, Sekine Y, Matsunaga N, Uchida Y, Takashima Y: Association of life-style with intraocular pressure in middle-aged and older Japanese residents. Jpn J Ophthalmol. 2003, 47 (2): 191-198. 10.1016/S0021-5155(02)00666-4.View ArticlePubMed
- Lee AJ, Rochtchina E, Wang JJ, Healey PR, Mitchell P: Does smoking affect intraocular pressure? findings from the blue mountains Eye study. J Glaucoma. 2003, 12 (3): 209-212. 10.1097/00061198-200306000-00005.View ArticlePubMed
- Avitabile T, Longo A, Rocca D, Amato R, Gagliano C, Castaing M: The influence of refractive errors on IOP measurement by rebound tonometry (ICare) and Goldmann applanation tonometry. Graefes Arch Clin Exp Ophthalmol. 2010, 248 (4): 585-591. 10.1007/s00417-009-1176-5.View ArticlePubMed
- Ehlers N: On corneal thickness and intraocular pressure. II. A clinical study on the thickness of the corneal stroma in glaucomatous eyes. Acta Ophthalmol (Copenh). 1970, 48 (6): 1107-1112.View Article
- Kohlhaas M, Boehm AG, Spoerl E, Pursten A, Grein HJ, Pillunat LE: Effect of central corneal thickness, corneal curvature, and axial length on applanation tonometry. Arch Ophthalmol. 2006, 124 (4): 471-476. 10.1001/archopht.124.4.471.View ArticlePubMed
- Zeppieri M, Brusini P, Miglior S: Corneal thickness and functional damage in patients with ocular hypertension. Eur J Ophthalmol. 2005, 15 (2): 196-201.PubMed
- Jensen JA, Goodson WH, Hopf HW, Hunt TK: Cigarette smoking decreases tissue oxygen. Arch Surg. 1991, 126 (9): 1131-1134. 10.1001/archsurg.1991.01410330093013.View ArticlePubMed
- Jorgensen LN, Kallehave F, Christensen E, Siana JE, Gottrup F: Less collagen production in smokers. Surgery. 1998, 123 (4): 450-455. 10.1016/S0039-6060(98)70167-9.View ArticlePubMed
- Melamed S, Ben-Sira I, Ben-Shaul Y: Corneal endothelial changes under induced intraocular pressure elevation: a scanning and transmission electron microscopic study in rabbits. Br J Ophthalmol. 1980, 64 (3): 164-169. 10.1136/bjo.64.3.164.PubMed CentralView ArticlePubMed
- Knuutinen A, Kokkonen N, Risteli J, Vahakangas K, Kallioinen M, Salo T, Sorsa T, Oikarinen A: Smoking affects collagen synthesis and extracellular matrix turnover in human skin. Br J Dermatol. 2002, 146 (4): 588-594. 10.1046/j.1365-2133.2002.04694.x.View ArticlePubMed
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2415/12/59/prepub
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