Cholesterol-enriched diet increased GFAP expression in retina. A. Cholesterol-fed rabbit retinal sections showed an increase in the expression of GFAP (Red). DAPI (blue) is a nuclear counter stain. B. Western blot results also showed elevated GFAP levels. ONL, INL, GCL are outer nuclear layer, inner nuclear layer and ganglion cell layers respectively. ***p < 0.001 vs control. Bar = 20 μm.