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Figure 2 | BMC Ophthalmology

Figure 2

From: DPOFA, a Cl-/HCO3 -exchanger antagonist, stimulates fluid absorption across basolateral surface of the retinal pigment epithelium

Figure 2

Expression analysis of Cl - /HCO3 - exchanger isoforms in human and bovine RPE. RT-PCR amplification was conducted on human (A) and bovine (C) RPE cDNA preparations. Panels Band Dshow the results of control PCR amplification with human (B) and bovine (D) isoform-specific primers performed with water in place of cDNA. Lanes 1-6 indicate PCR reactions performed with human (panels A, B) and bovine (panels C, D) oligonucleotides specific for SLC4A1, SLC4A2, SLC4A3, SLC26A3, SLC26A4, SLC26A6, respectively. Lane 7 represents amplification of the control GAPDH fragment performed with human (panels A, B) or bovine (panels C, D) oligonucleotide primers. Lane 8 contains size markers (100 b.p ladder) with arrow indicating a position of the 500 b.p. band.

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BMC Ophthalmology

ISSN: 1471-2415

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