Figure 3

Comparison of changes in water pumping rates in bovine RPE-choroid explants induced by DPOFA and vehicle treatment. DPOFA at 1-20 μM concentrations was added to apical and basal baths of the chamber. The number of experiments and change in pumping rate values are shown in Table 1. Mean values are plotted with error bars depicting Standard Error of Means (SEM). The change in pumping rate from apical to basal side of the RPE-choroid explant was calculated at 10, 15, 20, and 30 minutes after drug and vehicle addition. While no statistically significant difference between compound doses could be discerned, we were able to detect the statistically significant (p = 0.013) increase in fluid absorption after drug treatment versus vehicle control during the first 20 minutes post drug/vehicle addition when the data for all DPOFA concentrations within the 1-20 μM were pooled for the analysis.