Regionalization of S-cones in rd7 retina. (a) Staining of PNA (red), S-Opsin (green) and nuclei (blue) on a section of rd7 retina at PN35 (Scale bar: 100 μm). (d, b) Higher expression of S-opsin ventrally than dorsally at higher magnification. (e, c) The difference of expression of S-Opsin expression in wild type (C3HfHeA) at high magnification. Staining of PNA (f, h) and S-Opsin (g, i) in rd7 flat-mounted retina at PN35. Scale bar: 25 μm for b-i. (j) Virtual eye fundus representing the S-cone density in the rd7 retina in 9 rows from the centre (optic nerve). The density of S-cones in each segment is encoded by a color scale. Excluded areas are represented by a grey color and the absolute cone density value from dark blue, yellow to dark red are: 45, 90, 135, 180, 225, 270, 315, 360 and 405 cones/surface unit.