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Table 1 Microorganisms and postinoculation time points tested in this protocol and required by EP-A and USP/JP [11–13]

From: Effectiveness of ophthalmic solution preservatives: a comparison of latanoprost with 0.02% benzalkonium chloride and travoprost with the sofZia preservative system

Microorganisms included in all tests

Pseudomonas aeruginosa

Staphylococcus aureus

Candida albicans

Aspergillus brasiliensis

Escherichia coli*

Time points

Protocol

EP-A

USP/JP

0 hour

T

T

T

6 hours

T

T

N

24 hours

T

T

N

7 days

T

T (mold only)

T

14 days

T

N

T

28 days

T

T

T

  1. JP: Japanese Pharmacopoeia; N: not included in test; T: included in test; USP: United States Pharmacopeia.
  2. *By European Pharmacopoeia-A (EP-A) standards, only required for oral products [11].