Table 1 Primer sequence for real time RT-PCR
From: Role of Heat Shock Protein 47 in Transdifferentiation of Human Tenon's Fibroblasts to Myofibroblasts
Gene Name | Type | Sequence |
|---|---|---|
Hsp47 | Forward | 5’-CGC CAT GTT CTT CAA GCC A-3’ |
Reverse | 5’-CAT GAA GCC ACG GTT GTC C-3’ | |
αSMA | Forward | 5’-GTG TTA TGT AGC TCT GGA CTT TGA AAA-3’ |
Reverse | 5’-GGC AGC GGA AAC GTT CAT T-3’ | |
β-actin | Forward | 5’-GCG GGA AAT CGT GCG TGA CAT T-3’ |
Reverse | 5’-GAT GGA GTT GAA GGT AGT TTC GTG-3’ |