Figure 5

Confirmation western blotting using purified proteins for representative patients’ sera. Two hsp60s were used with sera presented in Figure 2. (A) Recombinant human hsp60 and (B) recombinant M. bovis Hsp65 (native) proteins (member of the hsp60 family of heat shock proteins); Note that some AAbs recognized only hsp65. (C) Recombinant CRMP-2 incubated with 15 patients’ sera that were suspected to react with CRMP-2. Patients’ AAbs bound to different molecular forms of CRMP-2, suggesting they recognized different epitopes. We used specific anti-human antibodies against CRMP-2 as positive control.