Figure 1

Cytotoxicity of H₂O₂ on human RPE cells. Fig. 1A: Primary human RPE cells were plated in 96-well plate, fed with serum-free medium the next day, then treated with H₂O₂ for 4 hours (circles) or 24 hours (squares). Fig. 1B: ARPE-19 cells were plated in 96-well plate, fed with serum-free medium the next day, then treated with H₂O₂ for 4 hours (circles) or 24 hours (squares). Fig. 1C: ARPE-19 cells were plated with a density of 10,000 cells/well, 20,000 cells/well or 40,000 cells/well in 96-well plates, fed with serum-free medium the next day, then treated with H₂O₂ for 24 hours. The viability of each treatment was determined by the MTT assay. Cultures at a density of 40,000 cells/well were 100% confluent.