Figure 3

Pax6 proteins activate expression from a reporter consisting of four copies of a PAX6-binding site found in the putative 5' flanking sequence of the human PALM gene cloned upstream of the E4 basal promoter. (A) An alignment between the PAX6 site found in the PALM gene and a consensus paired domain Pax6-binding site, P6CON. Non-conserved nucleotides are shown in lower case letters. (B) Results of co-transfections in 293T cells. 200 ng of Pax6 and 25 ng of Pax6(5a) expression plasmids were used as indicated per experiment. The data were normalized using Renilla luciferase [31] and are expressed as a relative ratio of promoter activity in the presence of Pax6 compared to the presence of empty vector, pKW10.