Figure 1

CD133 expression within adult human retinal and following magnetic CD133 cell sorting. (A) Frozen semi-thin retinal sections demonstrating CD133 expression within the photoreceptor layer (green) X400. Discrete CD133⁺ cells are also identified within the inner nuclear layer (INL, red arrow A) and ganglion cell layer (GCL, red arrow A). Panel B concentrates on the OPL and GCL X400 and C represents magnification of the GCL X630 within adult human retina. red arrows identify CD133⁺ cells (D) isotype control. Actin was labelled with rhodamine phalloidin and nuclei were counterstained with DAPI. Scale bars represent 0.5 μm (A, B, D) and 0.1 μm (C). Magnetic bead tagged -CD133 was used to isolate CD133⁺ cells from primary retinal cell suspension via positive selection (E) CD133⁺ expression was confirmed by flow cytometry in primary retinal cell culture (~67%) and is represented here as a FACS dot plot (grey) and following CD133⁺ purification.(~96% purity) cells display varying extent of expression (black). (F) Histogram to show the increase in CD133⁺ cells (black line) following magnetic separation from primary suspension (dotted line). The Grey filled peak represents isotype mAb fluorescence (0.82%).