Fig. 3

Protective effects of 14-3-3 antibody on RGC-5 stressed with H₂O₂. RGC-5 cells were preincubated with different concentration of 14-3-3 antibodies for 3 h and additionally stressed with 50 μM H₂O₂ for 1 h. This amount of H₂O₂ was used, as we did not want to produce a loss of viability through H₂O₂ our aim was to just increase ROS in the cells. The graph shows the ROS levels in % in the cells (blue line) as well as the viability (red line) of the cells after pre-incubation with different 14-3-3 antibody concentrations and stress with 50 μM H₂O₂ for 1 h. N in each experimental group is 4. Significantly decreased ROS levels (−31 %) were measured in the cells incubated with 10 μg/ml 14-3-3 antibodies. ROS-production was measured using DCFH-DA and expressed as percent of the control cells, which were only treated with H₂O_2. Significantly increased viability (+22 %) was measured for the cells incubated with 10 μg/ml 14-3-3 antibodies. Viability was measured using crystal violet and expressed as percent of the control cells additionally incubated with H₂O₂ (* = p < 0.05; **p < 0.01)