Table 1 Summary of the studies on MSC differentiating into corneal cells
From: Mesenchymal stem cells for treating ocular surface diseases
Cornea cell Differentiation | MSC type | In vitro | In vivo | Reference | ||
|---|---|---|---|---|---|---|
Method | Differentiation test | Method | Differentiation test | |||
Epithelium | Human BMMSC | No | No | Rat alkali burn model received BMMSC on AM | Human Krt3 (−); human keratin-pan (−) | [37] |
| Â | Rabbit BMMSC | Coculture with Rab-LSC or Rab-LSC conditioned medium | Krt3 (+) | Rabbit alkali burn model received BMMSCs on fibrin gel | Krt3 (+) | [38] |
| Â | Rat BMMSC | Coculture with rat corneal stromal cell | Krt12 (+) | Rat alkali burn model received induced MSCs on AM | Clinical assessment; Krt12 (+) | [39] |
| Â | Human ATMSC | Coculture with basal culture medium conditioned with human corneal epithelial cells | Krt3 (+); Krt12 (+) | No | No | [40] |
| Â | Human BMMSC | Sphere culture treated with RA, BMP4 and EGF followed by the cell dissociation and Matrigel culture | Krt3 (+); Krt12 (+); Krt8(+); Transepithelial Electrical Resistance test | No | No | [41] |
Keratocyte | Human UMSC | No | No | Kera−/− mouse and lum−/− mouse received UMSC corneal injection | Human keratocan (+); Lumican (+); CD34 (+); ALDH3A1 (+) | [44] |
|  | Mouse BMMSC | No | No | Kera−/− mouse received BMMSC corneal injection | Human keratocan (+) | [45] |
| Â | Human BMMSC | Cultured in human keratocyte conditioned medium | Human keratocan (+); Lumican (+); ALDH1A1 | No | No | [46] |
Endothelium | To be studied | To be studied | To be studied | To be studied | To be studied | Â |