Table 1 Experimental details for immunohistochemical (double) stainings for MMPs/TIMP and retinal cell markers on retinal cryosections and retinal flatmounts
| Â | Primary antibody (dilution) | Primary antibody supplier | Secondary antibody (dilution) |
|---|---|---|---|
Immunohistochemical staining for MMPs on retinal cryosections | |||
 MMP-2 | asc-8835-R (1:200) (ab1) | Santa Cruz, Dallas, TX | GAR-B (1:300) |
ab19167 (1:200) (ab2) | Millipore, Billerica, MA | GAR-B (1:300) | |
 MMP-3 | ab52195 (1:200) (ab3) | Abcam, Cambridge, United Kingdom | GAR-B (1:100) |
sc-6839-R (1:50) (ab4) | Santa Cruz, Dallas, TX | GAR-B (1:300) | |
 MMP-9 | ab58803 (1:100) (ab5) | Abcam, Cambridge, United Kingdom | GAM-B (1:300) |
ab38898 (1:500) (ab6) | Abcam, Cambridge, United Kingdom | GAR-B (1:300) | |
 MT1-MMP | ab53712 (1:200) (ab7) | Abcam, Cambridge, United Kingdom | GAR-B (1:300) |
RP-3 (1:200) (ab8) | Triple Point, Forest Grove, OR | GAR-HRP (1:300) | |
 TIMP-1 | a sc-5538-R (1:100) | Santa Cruz, Dallas, TX | GAR-B (1:300) |
Double staining for MMPs and retinal cell markers on retinal cryosections | |||
 MMP-2 | ab19167 (1:200) | Millipore, Billerica, MA | GAR-HRP (1:300) |
 + GS | MAB302 (1:500) | Millipore, Billerica, MA | GAM-Alexa (1:200) |
 MMP-3 | sc-6839-R (1:50) | Santa Cruz, Dallas, TX | GAR-HRP (1:300) |
 + Brn3a | MAB1585 (1:100) | Millipore, Billerica, MA | GAM-HRP (1:300) |
 MMP-3 | ab52195 (1:200) | Abcam, Cambridge, United Kingdom | GAR-HRP (1:300) |
 + GS | MAB302 (1:500) | Millipore, Billerica, MA | GAM-Alexa (1:200) |
 MMP-9 | ab58803 (1:100) | Abcam, Cambridge, United Kingdom | GAM-B (1:300) |
 + Iba-1 | 019-19741 (1:1000) | Wako, Osaka, Japan | GAR-Alexa (1:200) |
 MT1-MMP | ab53712 (1:200) | Abcam, Cambridge, United Kingdom | GAR-HRP (1:300) |
 + GS | MAB302 (1:500) | Millipore, Billerica, MA | GAM-Alexa (1:200) |
 MT1-MMP | RP-3 (1:200) | Triple Point, Forest Grove, OR | GAR-HRP (1:300) |
 + GS | MAB302 (1:500) | Millipore, Billerica, MA | GAM-Alexa (1:200) |
(Double) staining for MMPs and/or retinal cell markers on retinal flatmounts | |||
 MMP-9 | ab38898 (1:500) | Abcam, Cambridge, United Kingdom | DAR-B (1:300) |
 + Brn3a | sc-31984 (1:750) | Santa Cruz, Dallas, TX | DAG-Alexa (1:200) |
 MT1-MMP | ab53712 (1:200) | Abcam, Cambridge, United Kingdom | GAR-B (1:300) |
 + RT-97 | RT-97 (1:200) | Developmental Studies Hybridoma Bank, University of Iowa | GAM-Alexa (1:200) |
 MMP-2 | sc-8835-R (1:200) | Santa Cruz, Dallas, TX | GAR-Alexa (1:500) |
 GFAP | Z0334 (1:2000) | Dako, Glostrup, Denmark | GAR-Alexa (1:500) |