Fig. 3
Oxidant-Antioxidant Imbalance in Mice Corneal Endothelium of Dysfunctional Allografts a Three-dimensional profile of Mouse Oxidative Stress and Antioxidant Defense RT2-PCR Arrays. b Scatter plot of expression differences among genes related to the oxidative stress and antioxidant defense. c and d Semiquantitative Western blot analyses were conducted to determine the relative protein level of the five genes found by PCR array analysis to be expressed at significantly different levels in corneal endothelium of allogenic and syngenic corneal grafts. Fig. 3c shows representative blots for each down-expressed protein and Fig. 3d shows representative blots for each up-expressed protein and the corresponding GAPDH bands. The syngenic group was used as control. For each sample, the relative abundance of the protein of interest is determined by calculating the ratio of the intensity of the signal for the protein of interest to that of the normalization control GAPDH. Band densities determined by ImageJ software and compared with syngenic group. Three additional experiments achieved equivalent results. Data are means ± SD (n = 3). All means marked with *(t-test, P < 0.05) are significantly different from the control