Fig. 5

SA-β-Gal staining, gene expression profiling and microarray-based GO analysis of differentially expressed genes in human endothelium of dysfunctional corneal allografts Representative results of SA-β-Gal staining on human corneal endothelium in normal human corneal endothelium a and in dysfunctional allografts b. Compared with normal human corneal endothelium, SA-β-Gal positive cells were observed on human corneal endothelium in dysfunctional allografts. These results were consisted with that in the mice model. The result of gene expression profiling was shown as the scatter plot in Fig. 5-c. The scatter plot is a visualization that is useful for assessing the variation between human corneal endothelium in dysfunctional allografts (Y-axis) and in normal human corneal endothelium (X-axis). Microarray-based GO analysis of differentially expressed genes on human corneal endothelium between dysfunctional allografts and normal human corneal endothelium were shown in Fig. 5-d (the most significantly down-regulated genes) and Fig. 5-e (the most significantly up-regulated genes)