Fig. 2

Expression of CD147 and MMP-2 in a primary uveal melanoma sample analyzed by fluorescence double-immunohistochemistry. a A mosaic image was constructed from n = 96 images acquired at 50X magnification to demonstrate the expression of CD147 (green) and MMP-2 (red) in the whole tumor (67 year old female patient who developed metastasis after 6 years of follow-up). The nuclei were counterstained with DAPI. The upper white square on the merged image demonstrates the approximate location of the images which are presented in panels B and D. The lower white square demonstrates the approximate location of the images presented in panel C. R: Retina, C: Choroid. Scale bar: 2 mm. b, c Focused images acquired at 400X magnification, which demonstrate a strong expression of CD147 and MMP-2 at the tumor margin, and a weaker expression in the inner regions, respectively. Scale bars: 25 μm. d Images of the negative control which was performed by omitting the primary polyclonal CD147 and MMP-2 antibodies. Scale bars (a-d): 25 μm. e Expression of Endoglin and vWF in the same tumor sample. Scale bars: 100 μm