Fig. 4

Immunofluorescence microphotography (400x magnification field of view) of (a) a spindle cell melanoma of a 69 year old female patient (LTD: 5 mm) revealed clustered CD147 positive cells which were associated with MMP-2 up-regulation. No metastasic disease was detected clinically during a follow-up of 9 years. In a mixed cell uveal melanoma (b) nested CD147 positive cells were detected which were also associated with MMP-2 upregulation. This 90 year old patient presented with metastastic disease one year after enucleation. c No signals were detected in the negative controls performed by omitting the primary polyclonal CD147 and MMP-2 antibodies. Scale bar: 25 μm