CD147 and matrix-metalloproteinase-2 expression in metastatic and non-metastatic uveal melanomas

BMC Ophthalmology

Table 2 Summary of the applied primary and secondary antibodies

Primary antibodies
Antigen	Source	Dilution	Clone	Manufacturer	Pre-treatment	Visualisation
CD147	Mouse	1:50	P	Abcam, Cambridge, UK	Cooked in citrate puffer	Fluorescence (Alexa Fluor® 488)
MMP-2	Rabbit	1:100	P	Abcam, Cambridge, UK	Cooked in citrate puffer	Fluorescence (Cy3)
CD34	Rat	1:100	Clone MEC 14.7	Abcam, Cambridge, UK	Cooked in citrate puffer	Fluorescence (Cy5)
CD68	Rat	1:100	Clone FA-11	Abcam, Cambridge, UK	Cooked in citrate puffer	Fluorescence (Cy5)
GFAP	Chicken	1:1000	P	Abcam, Cambridge, UK	Cooked in citrate puffer	Fluorescence (Alexa Fluor® 647)
Endoglin	Mouse	1:5	Clone SN66	Dako, Glostrup, Denmark	100 mg Protease in 200 ml TBS	Chromogenic (HRP, DAB)
vWF	Mouse	1:50	Clone F8/86	Dako, Glostrup, Denmark	100 mg Protease in 200 ml TBS	Chromogenic (HRP, DAB)
CD31	Mouse	1:40	Clone JC704	Dako, Glostrup, Denmark	Cooked in TBS	Chromogenic (HRP, DAB)
Secondary antibodies
Reactivity	Source	Dilution	Clone	Conjugation	Manufacturer
Mouse IgG (H + L)	Goat	1:50	P	Alexa Fluor® 488	Molecular Probes®- Invitrogen, Eugene,USA
Rabbit IgG (H + L)	Goat	1:50	P	Cy3	Dianova, Hamburg, Germany
Rat IgG (H + L)	Donkey	1:50	P	Cy5	Dianova, Hamburg, Germany
Chicken IgY (H + L)	Goat	1:50	P	Alexa Fluor® 647	Dianova, Hamburg, Germany
Mouse IgG	Goat	1:250	P	HRP	Dianova, Hamburg, Germany

DAB 3,3′-Diaminobenzidine, HRP Horseradish peroxidase, P Polyclonal, TBS Tris-buffered saline

ISSN: 1471-2415