Fig. 6

Real-time PCR analyses and Western blot analyses of TANK-binding kinase (TBK)1 and IkappaB kinase-epsilon (IKK-ε) in human corneal fibroblast (HCF) cells with or without angiogenin (ANG) treatment. a The relative mRNA level of TBK1 was decreased by ANG in HCFs stimulated with tumor necrosis factor-alpha (TNF-α), and not in those stimulated with lipopolysaccharide (LPS); and the relative mRNA level of IKK-ε was diminished by ANG in HCFs stimulated either with TNF-α or LPS. b ANG treatment reduced TBK1 protein expression in HCFs stimulated with TNF-α, and not in those stimulated with LPS, and down-regulated IKK-ε protein expression in HCFs stimulated either with TNF-α or LPS. c Densitometric analysis of the expression of TBK1 and IKK-ε protein relative to beta-actin in HCFs. The experiments were repeated at least three times. One-way ANOVA followed by Bonferroni’s post hoc analysis, *p < 0.05