Table 1 Sequences of PCR primers
Gene | Sense primer (5’→3’) | Anti-sense primer (3’→5’) | Product size (bp) |
|---|---|---|---|
GAPDH | CGAGATCCCTCCAAAATCAA | TGTGGTCATGAGTCCTTCCA | 294 |
IL-1β | CCTGTCCTGCGTGTTGAAAGA | GGGAACTGGGCAGACTCAAA | 150 |
IL-4 | AACACAACTGAGAAGGAAACCTTC | GCTCGAACACTTTGAATATTTCTC | 276 |
IL-6 | TTCGGTCCAGTTGCCTTCTC | GAGGTGAGTGGCTCTCTGTG | 112 |
IL-8 | ACATGACTTCCAAGCTGGCCG | TTTATGAATTCTCAGCCCTC | 302 |
IL-10 | GCCTAACATGCTTCGAGATC | TGATGTCTGGGTCTTGGTTC | 206 |
TLR4 | AGCCTAAGCCACCTCTCTACCT | AGATTTGTCTCCACAGCCACCA | 116 |
MYD88 | ATGGTGGTGGTTGTCTCTGATG | GACAGGATGAACCTCAGGATGC | 165 |
TNFR1 | GTGCTGTTGCCCCTGGTCAT | GCTTAGTAGTAGTTCCTTCA | 163 |
TNFR2 | AAACTCAAGCCTGCACTC | GGATGAAGTCGTGTTGGAGA | 209 |
C1r | CAACAACTTTGAAACAACCA | GGAGAAGTCTGTGTGGAAGG | 237 |
C1s | AAGTCAGACTTTTCCAATGA | ACTTGCAATCTCCCCAATCA | 247 |
C2 | TGGAGTGGACAAGCTGTGCCG | TGAAAGTCTCGTGGCGGCGG | 289 |
C3 | TGGCTGTTCGCACCCTGGAT | AGCCCGAGGGGGTCACAATGA | 204 |
C4 | ATGGTTCCTATGCGGCTTGGTTGTC | GCGATGGTCACAAAGGCTGTGAGTG | 256 |
C5 | TGGCAACCAGCTCCAGGTTCAT | TGCCCCACAGCCCAGATCACT | 208 |
CFB | CAAGAAGGCCCTCCAGGCAGT | AAGGCCCGACCCCAAACACAT | 233 |
TBK1 | TTCTGGAAGTCCATACGCAT | ACTGGTGATCTCTATGCTGT | 237 |
IKK-ε | CTGCTCATGAATGACAGTGA | GGCGAGTGTATGTTATGCTT | 132 |