Fig. 3

Western blot analysis of Iba1 and CD68 3 days after establishment of acute ischemia. Differences in loading were normalized to the level of beta-actin. a, b Low expression of both Iba1 and CD68 is seen in the control and 1.0 mg/kg etanercept-treated ischemic groups, while distinct protein bands corresponding to Iba1 and CD68 are seen in the untreated ischemic group. c, d Levels of Iba1 and CD68 relative to beta-actin. a, c Iba1. b, d CD68. Cont, sham-treated control model; Ischemia, untreated ischemic group; Ischemia + T, ischemia with 1.0 mg/kg etanercept treatment group; B actin, beta-actin