Fig. 5

Histological sections of dystrophic corneal button from III:10 (a, b and C) and III:12 (d and e), and normal residue donor cornea (f). a Hematoxylin and Eosin staining (40X). b Higher magnification image (100X) of dystrophic aggregates presenting around the stromal keratocytes (pointed by black arrows). The region demarcated by the black rectangle in panel (a) is shown. c Masson-Trichrome staining (40X). d Hematoxylin and Eosin staining (40X). e Immunolabelling for TGFBI (40X). Black asterisks indicate dystrophic deposits stacking and producing three to five bands in superficial corneal layers. Black arrowheads point dystrophic aggregates scattering throughout the mid- to posterior stroma