Table 1 Lists of primers and PCR conditions used for amplification of TGFBI
Exon | Sequence of primers (5′ → 3′) | Product length (bp) | Annealing temperature (°C) |
|---|---|---|---|
4 | CCCCAGAGGCCATCCCTCCT (sense) CCGGGCAGACGGAGGTCATC (antisense) | 225 | 58 |
11 | GCCTTAATAACCCATCCCAGTGT (sense) TTTTCCCCATCCCAAGTCT (antisense) | 427 | 62 |
12 | CATTCCAGTGGCCTGGACTCTACTATC (sense) GGGGCCCTGAGGGATCACTACTT (antisense) | 318 | 55 |
13 | CCTCCTTGACCAGGCTAATTAC (sense) GGCTGCAACTTGAAGGTTGTG (antisense) | 299 | 58 |
14 | CTGTTCAGTAAACACTTGCT (sense) CTCTCCACCAACTGCCACAT (antisense) | 361 | 60 |