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Fig. 5 | BMC Ophthalmology

Fig. 5

From: Clinical implications of microbial biofilms in chronic rhinosinusitis and orbital cellulitis

Fig. 5

XTT reduction assay to show resistance patterns of fungal biofilm to amphotericin B. Panels show the MIC results of six Aspergillus biofilms (rows 1–6) in duplicate (Panel a and Panel b) showing amphotericin B MICs of 4,4,8,4,1 and 2 mg/L respectively in the rows 1, 2, 3, 4, 5 and 6. Strains in the row 3 with MIC value of 8 mg/L was a strong biofilm producing organisms and those in the rows 1,2 and 4 were weak biofilm producers as shown by the SEM study. Wells in the right most panels show positive controls without antifungal agent and those in the row below (labelled as “NC”) show the negative controls i.e. dye alone without any organisms exhibiting no reduction of the dye and thus no color intensity

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