Fig. 3
From: Neonatal disease environment limits the efficacy of retinal transplantation in the LCA8 mouse model
Section-antibody staining shows that transplanted eGFP (+) donor cells (green) differentiate into retinal cells in Pals1 CKO at P21. a – d Subsets of eGFP (+) cells (arrowheads in the box) express rhodopsin (red), and post-synaptic marker, PSD-95 (blue). e – h Subsets of donor cells (white arrowheads) differentiate into cells that express GS (red) and Sox 9 (blue), Muller glia markers. Examples of endogenous Muller cells are marked with yellow arrowheads. i – l Subsets of donors (arrows) express Chx10, progenitor/ bipolar cell marker (red). Images used in the insets are from one confocal scan while large images are maximally projected. White lines indicate subretinal space/inner and outer segments, ONL, INL and GCL (a & e, from top to bottom). Dysmorphic retinal morphology precludes definite identification of retinal layers in I. Hoechst 33258 staining (gray) is shown for nuclear counterstaining. Scale bars, 50 μm