Fig. 4

Transplanted eGFP (+) donor cells elicit mild host responses. a – d In normal retinal region of Pals1 CKO at P21, eGFP (+) cells recruit a small number of Iba I (+) cells (red, MG processes) and do not induce expression of CSPG (blue). e - h In degenerating retinal regions of Pals1 CKO at P21, small numbers of Iba I (+) MG cells (red) are recruited around transplanted cells while CSPG is not highly upregulated (blue, see below for comparison). Insets in f and g show high-mag views of an MG cell within the graft. i – l eGFP (+) donor cells recruit MG cells (red) and dramatically induce CSPG (blue) in host SW. Insets in j and k show MG cells located near transplanted eGFP(+) cells. Red lines indicate subretinal space/inner and outer segments, ONL, INL and GCL (from top to bottom). Hoechst 33258 staining (gray) is shown for nuclear counterstaining. Scale bars, 50 μm