Aqueous humor polymerase chain reaction in uveitis – utility and safety

BMC Ophthalmology

Table 1 PCR primers and probes sequences

HSV	Forward	5’- CCGTCAGCACCTTCATCGA -3’
	Reverse	5’-CGCTGGACCTCCGTGTAGTC -3’
	Probe	5’-CCACGAGATCAAGGACAGCGGCC-3’
VZV	Forward	5’- CGG CAT GGC CCG TCT AT -3’
	Reverse	5’-TCG CGT GCT GCG GC -3’
	Probe	5’-ATT CAG CAA TGG AAA CAC ACG ACG CC-3’
Toxo	Forward	5'- AGA GAC ACC GGA ATG CGA TCT - 3'
	Reverse	5'- CCC TCT TCT CCA CTC TTC AAT TCT – 3'
	Probe	5' -ACG CTT TCC TCG TGG TGA TGG CG -3'
EBV	Forward	5'- CGG AAG CCC TCT GGA CTT C -3’
	Reverse	5'- CCC TGT TTA TCC GAT GGA ATG -3’
	Probe	5'- TGT ACA CGC ACG AGA AAT GCG CC -3’
CMV	Forward	5'- GATCCGCTGACGCGTTTG-3’
	Reverse	5'- GCCGCCAGTCGTAACGAT-3’
	Probe	5'- TCATCGATCGGCGGATCACCAC -3’

The presence of foreign DNA was assessed by semi-quantitative rt-PCR. Table 1 demonstrates the PCR primers and probes sequences for HSV, VZV, CMV, EBV and Toxoplasma gondii that were used

ISSN: 1471-2415