Fig. 4

The rs7278468 G allele increases CRYAA transcription through enhancing Sp1 binding capacity. a Diagram of the CRYAA 5’UTR shows the Sp1 binding site within rs7278468. b ChIP analyses showing the rs7278468 G allele enhanced Sp1 binding. Right panel shows Sp1 binding to its responsive elements; left panel represents the control, which shows the DNA beyond Sp1 binding sites. Additionally, input is the positive control for the total genomic DNA extract before precipitation, and IgG is the negative control for nonspecific binding. A stronger PCR band was detected in the CRYAA_T-G-G transfected cells. c Levels of HLE cells alone and CRYAA_C-G-T and CRYAA_T-G-G transfected cells immunoprecipitated using Sp1 antibodies were measured following q-PCR. Signals from IgG controls used for each ChIP were subtracted from each of the Sp1 immunoprecipitation signals and the fold enrichment ratios of ChIP enriched vs total input were represented. The results are shown as the mean ± SD. (*p < 0.05). Gel photographs presented here are cropped and the originals are available in the Additional file 2: Figures S1 and S2)