Fig. 4
From: Notch1 signaling induces epithelial-mesenchymal transition in lens epithelium cells during hypoxia
Notch1/Snail1/E-cadherin pathway increased the invasion and migration ability of SRA01/04 cell. a-d WB image (a) and average relative band intensity of Notch1(b), Snail1 (c) and E-cadherin (d) showed the overexpression of Notch1 24 h after transfected with p3 × FLAG-CMV-7-NICD1 (group 3) and increased expression of Snail1 and decreased expression of E-cadherin compared with negative control either transfected with empty vector p3 × FLAG-CMV-7 (group 1) or non-transfected cells (group 2). e & g Representative images (g) and average number of invaded cells in the lower chamber during transwell assay 24 h after transfection of empty vector (group1), non-transfected (group2) and transfected with Notch1-NICD (group 3). f & h Representative images (h) and average scratch width in wound healing assay following the same transfection group as e & g